Australian Drosophila Transgenic Facility

This method provides the ability to achieve highly efficient and precise targeted integration of transgenes into the D. melanogaster  genome. By injecting a plasmid DNA construct containing an attB (bacterial attachment site) into specific fly strains already equipped with both an attP site (phage attachment site) and the phiC31 integrase enzyme, researchers can attain site-specific genetic modifications with remarkable accuracy.

Available landing sites:

• attP40 (white)
• attP2 (white)

Cost: $500 per transgenic line

Researchers have the option to utilise P-element transgenesis, a classical yet powerful technique for introducing DNA into the D. melanogaster genome. This method involves the use of modified P-elements that have been integrated in plasmid vectors alongside with transposase, allowing the random integration of transgenes. It's important to note that random integration may result in positional effects that exert an influence on the expression levels of transgenes.

Available strains: w1118.

Cost: $600 per transgenic line

Navigate to our iLab platform to initiate your order. If you are new, you may need to create an account.

Should you require any assistance, feel free to contact us or refer to our iLAB registration guidebook. 

Click on the "Request Services" tab to initiate your request for the desired transgenic service.

• After submitting your request, our team will review and generate a quote for the service.
• Update your payment information and include your Purchase Order number.
• You will then receive confirmation of your order.

Prepare and send in your plasmid DNA samples as per our guidelines. We recommend using a commercial kit and following the optional wash steps.

• Our team will commence the microinjection process upon receiving your plasmid samples.
• Once microinjection is completed, we will notify you.
• Expect your order to be completed within a timeframe of 8 weeks.

• Once the injected flies emerge, it will be backcrossed with their genetic background, and the resulting G1 generation will be screened for transformants.
• Following this, another round of backcrossing on the transformants (G1) will produce the G2 generation, which will be promptly delivered to you.