A/Prof Kaylene Simpson heads the Victorian Centre for Functional Genomics at Peter MacCallum Cancer Centre. The VCFG enables researchers Australia-wide to perform unbiased target discovery using high throughput approaches including CRISPR, RNAi and compound screening in both 2D and 3D, underpinned by sophisticated cell phenotyping using high content imaging. Kaylene also heads a quantitative protein expression profiling platform using Reverse Phase Protein Array technology.
Kaylene is a molecular cell biologist who specialised in breast cancer invasion and metastasis while a postdoctoral fellow at Harvard Medical School. She leads a highly experienced team who actively engage with researchers to help drive their research projects to fruition. She has a wealth of experience in assay development, data interpretation and analysis in the area of functional genomics and contributes intellectually to all projects and technology initiatives. She collaborates extensively both within Peter Mac and nationally and provides active grant support. She coordinates an annual national conference on Functional High Throughput Technologies, is a founding member and past President of the Society of Biomolecular Imaging and Informatics and is Senior Editor of Scientific DATA.
Chan KT, Blake B, Zhu H, Kang J, Trigos AS, Madhamshettiwar PB, Diesch J, Paavolainen L, Horvath P, Hannan RD, George AJ, Sanij E, Hannan KM, Simpson KJ and Pearson RB. A functional genetic screen defines the AKT-induced senescence signaling network. Cell Death and Differentiation. 2019.
Ma X, Zhang L, Song J, Nguyen E, Lee RS, Rodgers SJ, Li F, Huang C, Schittenhelm RB, Chan H, Cheang C, Wu J, Brown KK, Mitchell CA, Simpson KJ, Daly RJ. Characterization of the Src-regulated kinome identifies SGK1 as a key mediator of Src-induced transformation. Nature Communications. 2019
Lin HM, Nikolic I, Yang J, Castillo L, Deng N, Chan CL, Yeung NK, Dodson E, Elsworth B, Spielman C, Lee BY, Boyer Z, Simpson KJ, Daly RJ, Horvath LG, Swarbrick A. MicroRNAs as potential therapeutics to enhance chemosensitivity in advanced prostate cancer. Scientific Reports 2018 May 18;8(1):7820
Pearson H, Jason J, Meniel V, Fennell C, Waring P, Montgomery K, Rebello R, Macpherson A, Koushyar S, Furic L, Cullinane C, Clarkson R, Smalley M, Simpson KJ, Phesse T, Shepherd P, Humbert P, Sansom O and Phillips W. Identification of Pik3ca mutation as a genetic driver of prostate cancer that cooperates with Pten loss to accelerate progression and castration-resistant growth. Cancer Discovery 2018 Jun; 8(6):764-779
Cluse LA, Nikolic I, Knight D, Madhamshettiwar PB, Luu J, Cowley KJ, Semple T, Mir Arnau G, Shortt J, Johnstone RW and Simpson KJ (2018). A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens. Methods in Molecular Biology. 1725:201-227.
Nikolic I, Elsworth B, Dodson E, Wu SZ, Gould CM, Mestdagh P, Marshall GM, Horvath LG, Simpson KJ, Swarbrock A (2017). Discovering cancer vulnerabilities using high-throughput micro-RNA screening. Nucleic Acids Research. 45(22):12657-12670
Williams SP, Odell AF, Karnezis T, Farnsworth RH, Gould CM, Li J, Paquet-Fifield S, Harris NC, Walter A, Gregory JL, Lamont SF, Liu R, Takano EA, Nowell CJ, Bower NI, Resnick D, Smyth GK, Coultas L, Hogan Bm, Fox SB, Mueller SN, Simpson KJ, Achen MG, Stacker SA (2017). Genome-wide functional analysis reveals central signaling regulators of lymphatic endothelial cell migration and remodeling. Science Signaling. 10(499)
Manent J, Banerjee S, de Matos Simoes R, Zoranovic T, Mitsiades C, Penninger JM, Simpson KJ, Humbert PO, Richardson HE (2017). Autophagy suppresses Ras-driven epithelial tumourigenesis by limiting the accumulation of reactive oxygen species. Oncogene. 36(40):5576-5592
Haqshenas G, Wu J, Simpson, KJ, Daly RJ, Netter HJ, Baumert TF, Doerig C (2017). Signalome-wide assessment of host cell response to hepatitis C virus. Nature Communications. 8:15158
Williams SP, Gould CM, Nowell CJ, Karnezis T, Achen MG, Simpson KJ, Stacker SA (2017). Systematic high-content genome-wide RNAi screens of endothelial cell migration and morphology. Scientific Data. 4:170009
Foo CH, Rootes CL, Cowley K, Marsh GA, Gould CM, Deffrasnes C, Cowled CJ, Klein R, Riddell SJ, Middleton D, Simpson KJ, Wang LF, Bean AG, Stewart CR (2016). Dual microRNA Screens Reveal That the Immune-Responsive miR-181 Promotes Henipavirus Entry and Cell-Cell Fusion. PLoS Pathogens. 12(10):e1005974
Smith LK, Thomas DW, Simpson KJ, Humbert PO (2016). A Phenotypic High-Content Screening Assay to Identify Regulators of Membrane Protein Localization. Assay and Drug Development Technology. 14(8):478-488
Falkenberg KJ, Gould CM, Luu J, Matthews GM, Simpson KJ and Johnstone RW (2016). A genome scale siRNA screen reveals GLI1 as a novel gene regulating vorinostat sensitivity. Cell Death and Differentiation. 23(7):1209-18
Dutta B, Azhir A, Merino LH, Guo Y, Revanur S, Madhamshettiwar P, GermainRN, Smith JA, Simpson KJ, Martin SE, Beuhler E and Fraser ID (2016). CARD: an interactive web-based application for Comprehensive Analysis of RNAi-screen Data. Nature Communications.23;7:10578
Telford B, Chen A, Beethan H, Frick J, Brew T, Gould CM, Single A, Godwin T, Simpson KJ and Guilford P (2015). Synthetic lethal screens identify vulnerabilities in GPCR signalling and cytoskeletal organisation in E-cadherin-deficient cells. Molecular Cancer Therapeutics.14(5):1213-23