Associate Professor Kaylene Simpson Heads the Victorian Centre for Functional Genomics (VCFG) at Peter MacCallum Cancer Centre. The VCFG enables researchers Australia-wide to perform unbiased gene discovery using high throughput gene targeting approaches such as CRISPR/cas9 and RNA interference, quantitative Reverse Phase Protein Arrays, boutique compound screening and high content imaging. She leads a highly experienced team who actively engage with researchers to help drive their research projects to fruition.
Associate Professor Simpson is a molecular cell biologist who specialised in breast cancer invasion and metastasis while a postdoctoral fellow at Harvard Medical School. She has a wealth of experience in assay development, data interpretation and analysis and overall guidance in the area of functional genomics. She contributes intellectually to all projects and technology initiatives within the platform and collaborates with several Peter Mac Group Leaders to supervise PhD students.
Cluse LA, Nikolic I, Knight D, Madhamshettiwar PB, Luu J, Cowley KJ, Semple T, Mir Arnau G, Shortt J, Johnstone RW and Simpson KJ (2018). A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens. Methods in Molecular Biology. 1725:201-227.
Nikolic I, Elsworth B, Dodson E, Wu SZ, Gould CM, Mestdagh P, Marshall GM, Horvath LG, Simpson KJ, Swarbrock A (2017). Discovering cancer vulnerabilities using high-throughput micro-RNA screening. Nucleic Acids Research. 45(22):12657-12670
Williams SP, Odell AF, Karnezis T, Farnsworth RH, Gould CM, Li J, Paquet-Fifield S, Harris NC, Walter A, Gregory JL, Lamont SF, Liu R, Takano EA, Nowell CJ, Bower NI, Resnick D, Smyth GK, Coultas L, Hogan Bm, Fox SB, Mueller SN, Simpson KJ, Achen MG, Stacker SA (2017). Genome-wide functional analysis reveals central signaling regulators of lymphatic endothelial cell migration and remodeling. Science Signaling. 10(499)
Manent J, Banerjee S, de Matos Simoes R, Zoranovic T, Mitsiades C, Penninger JM, Simpson KJ, Humbert PO, Richardson HE (2017). Autophagy suppresses Ras-driven epithelial tumourigenesis by limiting the accumulation of reactive oxygen species. Oncogene. 36(40):5576-5592
Haqshenas G, Wu J, Simpson, KJ, Daly RJ, Netter HJ, Baumert TF, Doerig C (2017). Signalome-wide assessment of host cell response to hepatitis C virus. Nature Communications. 8:15158
Williams SP, Gould CM, Nowell CJ, Karnezis T, Achen MG, Simpson KJ, Stacker SA (2017). Systematic high-content genome-wide RNAi screens of endothelial cell migration and morphology. Scientific Data. 4:170009
Foo CH, Rootes CL, Cowley K, Marsh GA, Gould CM, Deffrasnes C, Cowled CJ, Klein R, Riddell SJ, Middleton D, Simpson KJ, Wang LF, Bean AG, Stewart CR (2016). Dual microRNA Screens Reveal That the Immune-Responsive miR-181 Promotes Henipavirus Entry and Cell-Cell Fusion. PLoS Pathogens. 12(10):e1005974
Smith LK, Thomas DW, Simpson KJ, Humbert PO (2016). A Phenotypic High-Content Screening Assay to Identify Regulators of Membrane Protein Localization. Assay and Drug Development Technology. 14(8):478-488
Falkenberg KJ, Gould CM, Luu J, Matthews GM, Simpson KJ and Johnstone RW (2016). A genome scale siRNA screen reveals GLI1 as a novel gene regulating vorinostat sensitivity. Cell Death and Differentiation. 23(7):1209-18
Dutta B, Azhir A, Merino LH, Guo Y, Revanur S, Madhamshettiwar P, GermainRN, Smith JA, Simpson KJ, Martin SE, Beuhler E and Fraser ID (2016). CARD: an interactive web-based application for Comprehensive Analysis of RNAi-screen Data. Nature Communications.23;7:10578
Telford B, Chen A, Beethan H, Frick J, Brew T, Gould CM, Single A, Godwin T, Simpson KJ and Guilford P (2015). Synthetic lethal screens identify vulnerabilities in GPCR signalling and cytoskeletal organisation in E-cadherin-deficient cells. Molecular Cancer Therapeutics.14(5):1213-23
Falkenberg KJ, Gould CM, Johnstone RW, Simpson KJ (2014).Genome-wide functional genomic and transcriptomic analyses for genes regulating sensitivity to vorinostat. Scientific Data.1:140017
Thomas DW, Gould CM, Handoko Y and Simpson KJ (2014). Functional genomics down under: RNAi screening in the Victorian Centre for Functional Genomics. Combinatorial Chemistry and High Throughput Screening Special Issue on Academic Screening Operations.17(4):343-355