CAHM Workshop content (Recordings available via our YouTube page) |
Intro to OPAL mIHC | An introduction to how to perform multiplex immunohistochemistry using OPAL staining |
FIJI for Beginners | Learn how to open and visualise microscopy data using FIJI |
FIJI for Quantification | Learn how to extract quantitative data using FIJI |
Online resources |
Spectraviewer | Look up excitation and emission spectra for each of your fluorophores (Thermo Fisher fluorophores) |
FPbase | Look up all details about fluorescent proteins (Ex, Em, EC, QY, Brightness, pKa, lifetime, photostability, sequence) |
Nyquist calculator | Work out the correct sampling rate for your experiment |
Analysis software |
Imaris | Online tutorials and webinars on how to use Imaris |
Volocity | Online tutorials on how to use Volocity |
Arivis | Online tutorials on how to use Arivis |
Halo/InForm | Akoya academy, webinars in sample prep and image analysis of mIHC |
Zeiss software | Download the lite version of Zen Blue or Zen Black |
Leica software | Download the lite version of LAS X |
Olympus | Download software for opening files from confocal and slide scanners |
Nikon | Download NIS Elements viewer |
FIJI (Image J) | Download FIJI for Windows, MacOS, Linux |
QuPath | Download QuPath |
Online learning |
iBiology Microscopy series |
MyScope Microscopy training |
Leica Science Lab |
Zeiss Education in Microscopy |
Nikon MicroscopyU |
Olympus Microscopy Resource Centre |