Mountford JK, Petitjean C, Putra HWK, McCafferty JA, Setiabakti NM, Lee H, Tonnesen LL, McFadyen JD, Schoenwaelder SM, Eckly A, Gachet C, Ellis S, Voss AK, Dickins RA, Hamilton JR, Jackson SP. The class II PI 3-kinase, PI3KC2α, links platelet internal membrane structure to shear-dependent adhesive function. Nat Commun. 2015; 6:6535.
Xing YL, Röth PT, Stratton JA, Chuang BH, Danne J, Ellis SL, Woei Ng S, Kilpatrick TJ, Merson TD. Adult neural precursor cells from the subventricular zone contribute significantly to oligodendrocyte regeneration and remyelination. J Neurosci. 2014; 34(42):14128-46.
Ellis SL, Heazlewood SY, Williams B, Reitsma AJ, Grassinger J, Borg J, Heazlewood CK, Chidgey AP, Nilsson SK. The role of Tenascin C in the lymphoid progenitor cell niche. Exp Hematol. 2013; 41(12):1050-61. (Note: selected as a Journal Highlight)
Susanto O, Stewart SE, Voskoboinik I, Brasacchio D, Hagn M, Ellis S, Asquith S, Sedelies KA, Bird PI, Waterhouse NJ, Trapani JA. Mouse granzyme A induces a novel death with writhing morphology that is mechanistically distinct from granzyme B-induced apoptosis. Cell Death Differ. 2013; 20(9):1183-93.
Steel R, Cross RS, Ellis SL, Anderson RL. Hsp70 architecture: the formation of novel polymeric structures of Hsp70.1 and Hsc70 after proteotoxic stress. PLoS One. 2012; 7(12):e52351.
Debrincat MA, Josefsson EC, James C, Henley KJ, Ellis S, Lebois M, Bettrman KL, Lane RM, Rogers KL, White MJ, Roberts AW, Harvey NL, Metcalf D, Kile BT. Mcl-1 and Bcl-xL coordinately regulate megakaryocyte survival. Blood. 2012; 119(24):5850-8.
Ellis SL, Nilsson SK. The location and cellular composition of the hemopoietic stem cell niche. Cytotherapy. 2012; 14(2):135-43.
Josefsson EC, James C, Henley KJ, Debrincat MA, Rogers KL, Dowling MR, White MJ, Kruse EA, Lane RM, Ellis S, Nurden P, Mason KD, O'Reilly LA, Roberts AW, Metcalf D, Huang DC, Kile BT. Megakaryocytes possess a functional intrinsic apoptosis pathway that must be restrained to survive and produce platelets. J Exp Med. 2011; 208(10):2017-31.
Brennan AJ, Chia J, Browne KA, Ciccone A, Ellis S, Lopez JA, Susanto O, Vershoor S, Yagita H, Whisstock J, Trapani JA, Vosboboinik I. Protection from endogenous perforin: glycans and the C terminus regulate exocytic trafficking in cytotoxic lymphocytes. Immunity. 2011; 34(6):879-92.
Ellis SL, Grassinger J, Jones A, Borg J, Camenisch T, Haylock D, Bertoncello I, Nilsson SK. The relationship between bone, hemopoietic stem cells, and vasculature. Blood. 2011; 118(6):1516-24.
Ellis SL, Williams B, Asquith S, Bertoncello I, Nilsson SK. An innovative triple immunogold labeling method to investigate the hemopoietic stem cell niche in situ. Microsc Microanal. 2009; 15(5):403-14.
Kruse EA, Loughran SJ, Baldwin TM, Josefsson EC, Ellis S, Watson DK, Nurden P, Metcalf D, Hilton DJ, Alexander WS, Kile BT. Dual requirement for the ETS transcription factors Fli-1 and Erg in hematopoietic stem cells and the megakaryocyte lineage. Proc Natl Acad Sci USA. 2009; 106(33):13814-9.
Paquet-Fifield S, Schlüter H, Li A, Aitken T, Gangatirkar P, Blashki D, Koelmeyer R, Pouliot N, Palatsides M, Ellis S, Brouard N, Zannettino A, Saunders N, Thompson N, Li J, Kaur P. A role for pericytes as microenvironmental regulators of human skin tissue regeneration. J Clin Invest. 2009; 119(9):2795-806.
Shackleton M, O'Reilly LA, Sutherland KD, Bath ML, Ellis S, Strasser A, Visvader JE, Lindeman GJ. Impaired lactation in mice expressing dominant-negative FADD in mammary epithelium. Dev Dyn. 2009; 238(4):1010-6.
Loughran SJ, Kruse EA, Hacking DF, de Graaf CA, Hyland CD, Willson TA, Henley KJ, Ellis S, Voss AK, Metcalf D, Hilton DJ, Alexander WS, Kile BT. The transcription factor Erg is essential for definitive hematopoiesis and the function of adult hematopoietic stem cells. Nat Immunol. 2008; 9(7):810-9.
Mason KD, Carpinelli MR, Fletcher JI, Collinge JE, Hilton AA, Ellis S, Metcalf D, Roberts AW, Huang DCS, Kile BT. Programmed anuclear cell death delimits platelet life span. Cell. 2007; 128(6):1173-86.
Articles of Interest
Mattheyses AL, Simon SM, Rappoport JZ (2010). Imaging with total internal reflection fluorescence microscopy for the cell biologist. J Cell Sci. 123(Pt 21):3621-8.
This article describes the biological applications and advantages of using total internal reflection fluorescence (TIRF) microscopy. The physical basis of TIRF microscopy is explained in detail, and TIRF experimental design and troubleshooting are commented on.
Renz M (2013). Fluorescence microscopy–A historical and technical Perspective. Cytometry. 83(9):767-79.
This review describes the basic principles and historic development of fluorescence microscopy. Confocal, spinning disc, two-photon and TIRF microscopy, FRAP, FRET and fluorescence correlation spectroscopy are reviewed. Developments in super resolution microscopy (SIM, STED, PALM, STORM) are also described.
North AJ (2006). Seeing is believing? A beginners' guide to practical pitfalls in image acquisition. J Cell Biol. 172(1):9-18.
A review outlining tips for achieving the best possible images.
Correlative light and electron microscopy
Padman BS, Bach M, Ramm G (2014). An improved procedure for subcellular spatial alignment during live-cell CLEM. PLoS One. 9(4):e95967.
A CLEM procedure has been developed that utilises toner particles from a laser printer as orientation markers for the precise spatial alignment of optical and electron microscopy data sets.
Super resolution microscopy
Carlson AL, Fujisaki J, Wu J, Runnels JM, Turcotte R, Spencer JA, Celso CL, Scadden DT, Strom TB, Lin CP (2013). Tracking single cells In live animals using a photoconvertible near-infrared cell membrane label. PLoS One. 8(8):e69257.
This article describes the photoconvertible properties of the dye DiR and how it can be used to track cell division of transplanted stem cells in live mice at the single cell level.
Chen BC, Legant WR, Wang K, et al. (2014). Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution. Science. 346(6208):1257998.
This article outlines a new super resolution microscopy technique using ultrathin sheets of light. This was conceived by Nobel laureate Eric Betzer.
Ma H, Yang Y, Roberts E, Yandl E (2008). Comparison of decalcification methods on rodent femurs. Histologic. XLI(2):32-5.
This article describes methods for bone decalcification prior to histological processing.
Jiang X, Kalajzic Z, Maye P, Braut A, Bellizzi J, Mina M, Rowe DW (2005). Histological analysis of GFP expression in murine bone. J Histochem Cytochem. 53(5):593–602.
This article provides an approach for recording multiple images from the same histological section that allows colocalisation of a GFP signal with subsequent stains and procedures that destroy GFP.
McDonald LG, Walker M, Bennett A (2008). Colorization of tissues with methylene blue as an alternative to eosin and erythrosin B. Histologic. XLI(2):36-7.
This article illustrates a method for staining tissues for visualisation while sectioning, without increasing autofluorescence.
Watson L, Vassallo J, Cantor G, Lehman-McKeeman L (2008). Lectin histochemistry: An alternative to immunohistochemistry for identifying specific structures in rat renal papillary necrosis. Histologic. XLI(2):28-31.
This article outlines the use of lectins as a tool for labelling specific carbohydrates in tissue structures.
Studer D, Graber W, Al-Amoudi A, Eggli P (2001). A new approach for cryofixation by high-pressure freezing. J Microsc. 203(Pt 3): 285-94.
This article describes the mechanics of the Leica EMPACT high-pressure freezer and its ability to vitrify and freeze specimens at a pressure of 2000 bar. A protocol for freeze substitution and resin embedding of samples is summarised.
Di Sant’Agnese PA, De Mesy-Jensen KL (1984). Diagnostic electron microscopy on reembedded (“popped off”) areas of large Spurr epoxy sections. Ultrastruct Pathol. 6(2-3):247-53.
A pop-off technique has been developed, in which small areas of thick Spurr epoxy sections cut from large blocks are removed from glass slides by inverted BEEM capsule “pop off” prior to ultrathin sectioning. Fixation, dehydration, embedding and pop-off procedures are described.
A/Prof Sarah Ellis
Phone: 03 8559 7822
Email: [email protected]