GENOTYPING CORE

The Genotyping Core facility offers researchers an efficient and cost-effective genotyping service, as well as cell line testing for mycoplasma contamination.

Genotyping Core equipment

The aim of the Genotyping Core is to provide support to research groups within Peter Mac, within the Victorian Comprehensive Cancer Centre (VCCC) and external to the VCCC. Our Parkville location is very convenient for researchers in the Parkville precinct.

We provide a comprehensive service, from preparation of genomic DNA from tissue samples all the way to reporting of genotypes (and upload of genotypes into your preferred database where possible).

The Genotyping team has more than 30 years’ collective expertise in mouse genotyping and has been providing genotyping services internally at Peter Mac for 12+ years. Now officially a Peter Mac Core facility, the Genotyping Core is keen to assist researchers external to Peter Mac through provision of convenient, timely, high-quality genotyping services, as well as cell line mycoplasma testing services.

SERVICES

Genotyping

  • Range of methods used:
    • PCR
    • qPCR, including Taqman / KASP assays / melt curve analysis
    • FACS (phenotyping to determine genotype)
  • Assay development and optimisation, free of charge
  • Affordable pricing includes testing of multiple genes

Mycoplasma testing

  • PCR-based test based on an established method (J Immunol Methods 1993; 164:91-100)
  • Detects 16S rRNA of more than 25 different mycoplasma species, including those most commonly found in cell cultures

KEY BENEFITS

  • Affordable and competitive pricing
  • Fast turn-around time – results within 5 working days
  • Convenient and local for the Parkville precinct:
    • Internal users / VCCC building partners:
      • Sample drop-off at Genotyping Core (for genotyping, Peter Mac animal technicians deliver samples directly)
      • Mycoplasma testing requests submitted via iLab
      • Genotype results uploaded to Tick @ Lab
      • Billing via iLab
    • External users:
      • Sample drop-off at VCCC, or send via Express Post
      • Testing requests submitted via iLab
      • Billing via iLab

CORE USE

Genotyping and cell line mycoplasma test services are available to all VCCC members and external users. For further information about pricing and sample submission please contact us.

THE TEAM

Janelle Sharkey (BAppSc): Genotyping Core Manager. Specialised in biology and biotechnology, with interest in immunology, cancer research and mouse models. Niche expertise: mouse genotyping via PCR, qPCR and FACS; genotyping assay development; primer design; mouse genetics.
[email protected]

Rebecca (Bec) D’Sylva (BBiomedSc(Hons)): Research Assistant. Specialised in biology and biotechnology, with interest in genetics, cancer research and mouse models. Niche expertise: mouse genotyping via PCR, qPCR and FACS; genotyping assay development; genotyping workflow automation.
[email protected]

Taryan Mathews (BHlth&BiomedSc(Hons)): Research Assistant. Specialised in biology and biotechnology, with interest in genetics, cancer research, molecular basis for disease and mouse modelling. Niche expertise: mouse genotyping via PCR, qPCR and FACS; genotyping assay development; primer design; mouse genetics.
[email protected]

CONTACT

Janelle Sharkey

Phone: (03) 8559 5075

Email: [email protected]

Team email: [email protected]